Why do we use cross adsorbed secondary antibodies?

Using cross adsorbed secondary antibodies is particularly useful for minimizing high background, non-specific binding, and multiplexing cross-reactivity with other primary antibodies and secondary antibodies when performing multiple labeling experiments or working with multiple primary antibodies.

What are cross-adsorbed secondary antibodies?

Cross-adsorbed secondary antibodies are polyclonal antibodies that are manufactured with an additional purification step to filter out members that bind to off-target species of immunoglobulin (IgG). The process decreases species cross-reactivity and increases specificity.

Should cross-adsorbed antibodies be used if cross-reactivity is not a concern?

For applications in which species cross-reactivity is not a concern, the use of cross-adsorbed antibodies is optional. The additional purification steps make highly cross-adsorbed antibodies more expensive, which may be a consideration.